AV无码久久久久不卡网站毛片,无码精品人妻一区二区三区漫画,久久精品国产99国产精品,全免费A级毛片免费看无码

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2547Panc 10.05 人胰腺腺癌細(xì)胞

Panc 10.05 人胰腺腺癌細(xì)胞

簡(jiǎn)要描述:CRL-2547 Panc 10.05 人胰腺腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2547
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-06-28
  • 訪  問  量:2132

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2547 Panc 10.05 人胰腺腺癌細(xì)胞

ATCC® Number: CRL-2547™ Price: $338.00

Designations: Panc 10.05

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2547 Panc 10.05 人胰腺腺癌細(xì)胞

Isolation: Isolation date: 1992

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II -

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 9,12

D5S818: 13

D7S820: 8,9

THO1: 6,9.3

TPOX: 11

vWA: 16

Age: adult

Gender: male

Ethnicity: White

Comments: Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.

Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 40%. [50655]

The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558).

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 19.2 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-2558

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602



CRL-2547
來自ATCC細(xì)胞,所有出入庫(kù)細(xì)胞均經(jīng)過嚴(yán)格的細(xì)胞質(zhì)量檢測(cè),確保細(xì)胞無污染,符合檢測(cè)合格標(biāo)準(zhǔn)。歡迎來詢價(jià)詳談


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
18禁美女挤奶视频免费观看| 成人H动漫精品一区二区| 9999国产精品欧美久久久久久| 内射人妻无码色AV无码| 无码精品人妻一区二区三区中| 欧美精品九九99久久在免费线| 天堂中文8资源在线8| 久久精品国产亚洲AV高清热| 色婷婷综合久久久中文字幕| 亚洲精品国精品久久99热| 宝贝我硬了~你含一下它| 亚洲精品巨爆乳无码大乳巨| 憋尿快崩溃的时候注水| 久久久国产精华液2023| 337P粉嫩日本亚洲大胆艺术| 性色AV夜夜嗨AV浪潮牛牛| 精品人妻无码一区二区三区手机版| 无人区码卡3卡4卡毛毛片| 国内露脸少妇精品视频| 久久影院午夜理论片无码| 美女无遮挡直播软件免费看| 无码AV中文一区二区三区桃花岛| 九九精品99久久久香蕉| 亚洲精品久久AV无码一区二区| 国精品人妻无码一区二区三区性色| 东北50岁熟妇露脸在线| 爆乳2把你榨干哦OVA在线观看| 亚洲av激情无码专区在线播放| 亚洲精品综合欧美一区二区三区| 精品国产污污免费网站AⅤ| 337P日本欧洲亚洲大胆色噜噜| 国产色婷亚洲99精品AV| 麻豆免费视频网站入口在线观看| 农民人伦一区二区三区| 少妇被三个黑人4P到惨叫| 97人妻人人澡人人爽国产| 口述他进我身体全过程| 日本做受高潮好舒服视频| 无遮挡H纯肉动漫在线播放| 黑人刚破完处就三P| 久久99国产精品久久99|